Evaluation of microbiological and physical parameters of water from dental clinics in a brazilian university

Authors

  • Fábio Barbosa Souza Federal University of Pernambuco
  • Fernanda Pimentel Malta Federal University of Pernambuco
  • Manoela Valadares de Souza Brandão Federal University of Pernambuco
  • Anna Cecília Farias Silva Federal University of Pernambuco
  • Beatriz Ribeiro Ribas Federal University of Pernambuco
  • Gláucia Manoella Souza Lima Federal University of Pernambuco

DOI:

https://doi.org/10.21615/cesodon.33.2.1

Keywords:

contamination, dentistry, dental offices, water

Abstract

Introduction and objective: Contamination of the water used in health care units can induce adverse individual and collective consequences. The objective of this study was to evaluate the water quality in dental clinics of a dentistry program of a university in Brazil, calculating the total coliforms, thermotolerant coliforms, heterotrophic bacteria and pH. Materials and methods: In each clinic, water samples were collected for analysis at different points: external cistern, clinic faucet, dental chair tank and triple syringe. After sample collection, analysis was performed: the presence of total coliforms and thermotolerant coliforms was determined by chromogenic substrate technique, the presence or absence of Escherichia coli by fluorescence in ultraviolet light, the count of heterotrophic bacteria through the number of colonies and the determination of pH. Results: In all studied sites, the presence of total coliforms, Escherichia coli and thermotolerant coliforms was observed in at least 26% of the samples. In relation to CFU / ml, all mean values were much higher than the maximum established by legislation (500 CFU / ml). It was verified a statistically significant difference was found in the external cistern and tap when compared to the chair tank and triple syringe (p < 0.05). All pH samples were within the standards. Conclusion: The global analysis showed that 100% of the samples were inadequate, which classified the water potability as nonstandard in regard to the current Brazilian legislation.

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References

Araújo CM, Lopes-Silva MAS. Análise da qualidade da água de reservatórios de equipamentos odontológicos. Rev Biociênc 2002; 8: 29-36.

Garg SK, Mittal S, Kaur P. Dental unit waterline management: historical perspectives and cu rrent trends. J Investig Clin Dent 2012; 3: 247-252.

Ajami B, Ghazvini K, Movahhed T, Ariee N, Shakeri M, Makarem S. Contamination of a dental unit water line system by legionella pneumophila in the mashhad school of dentistry in 2009. Iran Red Crescent Med J 2012; 14: 376-378.

Souza-Gugelmin MCM, Lima CDT, Lima SNM, Mian H, Ito IY. Microbial contamination in dental unit waterlines. Braz Dent J 2003; 14, 53-57.

Pinto K, Paula C. Protocolo de biossegurança no consultório odontológico: custo e tempo. Rev Biociênc 2003; 9: 19-23.

Goksay D, Cotuk A, Zeybek Z. Microbial contamination of dental unit waterlines in Istambul, Turkey. Environ Monit Assess 2008; 147: 265-269.

Pasquarella C, Veronesi L, Napoli C, Castiglia P, Liguori G, Rizetto R et al. Microbial environmental contamination in Italian dental clinics: a multicenter study yelding recommendations for stardardized sampling methods and threshold values. Sci Total Environ 2012; 420: 289-299.

Arvand M, Hack A. Microbial contamination of dental unit waterlines in dental practices in hesse, germanya cross-sectional study. Eur J Microbiol Immunol 2013; 3 49-52.

Hiran VMX, Bulla RJ, Mello LL, Moreno T, Tohnim MCB, Garcia LB. Análise bacteriológica da água de equipos odontológicos. Acta Sci 2000; 22: 631-636.

Brasil. 2011 Ministério da Saúde. Portaria n° 2.914. 12 de dezembro de 2011.

Gusmão ICCP, Gusmão JMR. Diagnóstico Ambiental e bacteriológico da água do equipo odontológico na cidade de Vitória da Conquista (BA). Estud Biol 2013; 35: 195-200.

Brasil. 2004. Ministério da Saúde. Portaria nº 518 de 25 de março de 2004. Estabelece os procedimentos e responsabilidades relativos ao controle e vigilância da qualidade da água para consumo humano e seu padrão de potabilidade, e dá outras providencias. Diário Oficial da União, Brasília, 26 mar. Seção 1, p. 266-270.

Franco DGMF, Landgraf M. Microbiologia dos alimentos. 2007; Atheneu, São Paulo.

Xavier HVM, Bulla J R, Luiza LM, Moreno T, Tognim MCB, Garcia LB. Análise bacteriológica da água de equipos odontológicos. Acta Sci 2000; 22: 631-636.

Pankhurst CL, Johnson NW. Microbial contamination of dental unit waterlines: the scientific argument. Int Dent J 1998; 48: 359-368.

Díaz, CG. La evaluación de la calidad microbiológica del agua em unidades dentales. Rev Cubana de Higiene y Epidemiologia 2009; 47: 1-10.

Claudino D, Lima AMT, Botega T A, Serratine ACP. Avaliação de Solução à Base de NaOCl 0,2% na Desinfecção das Tubulações de Água dos Equipos Odontológicos. Pesq Bras Odontoped Clin Integr 2011; 11: 471-476.

Watanabe A, Tamaki N, Yokota A, Matsuyama M, Kokeguchi S. Monitoring of bacterial contamination of dental unit water lines using adenosine triphosphate bioluminescence. J Hosp Infect 2016; 94: 393-396

Mills SE. Waterborne pathogens and dental waterlines. Dental Clin North Am 2003; 47: 545-57.

Murray JP, Slack GL. Some sources of bacterial contamination in everyday dental practice. Br Dent J 1957; 102: 172.

Galvão CF, Motta GF, Alvarez-Leite ME. Análise quantitativa da contaminação da água das tubulações e equipamentos odontológicos. Arq Bras Odontol 2006; 1: 3-9.

Petti S. Epidemiology and control in dental healthcare settings. Acta Stomatol Naissi 2013; 67: 1224-1229.

Centers For Disease Control and Prevention. Guidelines for Infection Control in Dental Health-Care Settings. MMWR. 2003; 52: 1-68.

Ada. Dental unit waterlines: approaching the year 2000. J Am Dent Assoc 1999; 130: 1653-1656.

Watanabe E, Pimenta FC, Agostinho AM, Matsumoto W, Ito IY. Diferentes métodos de avaliação do nível de contaminação microbiana da água de alta rotação. Robrac 2006; 15: 3-9.

Brasil. 2006 Ministério da Saúde. Secretaria de Vigilância em Saúde. Vigilância e controle da qualidade da água para consumo humano. Brasília: Ministério da Saúde. 212p.

Dobaradaran S, Nabipour I, Ramavandi B, Zazouli MA, Tahmasebi R, Ghaedi H et al. Microbial contamination of dental unit waterlines in Bushehr. Iran Fresen Environ Bull 2014; 23: 1000-1005.

Lizon J, Florentin A, Martrette J, Rivier A, Clement C, Rabaud C. Microbial Control of dental unit water: feedback on different disinfection methods experience. Am J Infect Control 2016; 44: 247-249.

Barbeau J. Waterborne Biofilms and Dentistry: The Changing Face of Infection Control. J Can Dent Assoc 2000; 66: 539-41.

Robert A, Bousseau A, Costa D, Barbot V, Imbert C. Are dentists enough aware of infectious risk associated with dental unit waterlines? Bull Group Int Rech Sci Stomatol Odontol 2013; 52: 29-34.

Tippet BF, Edwards JL, Howard FJ. Bacterial contamination of dental unit water lines-a possible source of cross-infection. N Z Dent J 1988; 84: 112-113.

Brasil. Serviços Odontológicos: Prevenção e Controle de Riscos/ Ministério da Saúde, Agência Nacional de Vigilância Sanitária. 1st ed. Brasília: Anvisa; 2006.

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Published

2020-12-31

How to Cite

1.
Souza FB, Malta FP, de Souza Brandão MV, Silva ACF, Ribas BR, Souza Lima GM. Evaluation of microbiological and physical parameters of water from dental clinics in a brazilian university. CES odontol. [Internet]. 2020 Dec. 31 [cited 2024 May 11];33(2):2-11. Available from: https://revistas.ces.edu.co/index.php/odontologia/article/view/4807

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Artículo de Investigación Científica y Tecnológica
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