Leukogram, total and differentiated proteins in young Equus mulus from the police in Boyacá (Colombia)

Authors

DOI:

https://doi.org/10.21615/cesmvz.6313

Keywords:

hematology, leukocytes, proteins, young mules, welfare

Abstract

Objective. To determine leukocyte, total protein, fibrinogen, albumin and globulin values in young mules belonging to the Colombian National Police. Matherials and Methods. 50 healthy male and female mules were sampled. Blood samples were obtained by jugular venipuncture for total and differential count analisis od leukocytes, albumin, globulin, and total plasma proteins by Neubauer counting chamber, blood smear and spectrophotometry. The data were analyzed by descriptive statistics, and a comparative analisis was performed with the mean reported for other equids using the T-student test (P<0.05) and the Wilcoxon test. A reliability of 95% with a margin of error 0f 5% were stablished. Results. Total White cell count: 11.32 ± 2.99 x 10⁹/L, differential count: neutrophils 5038.6 ± 2228 cells/µl, lymphocytes: 5758 ± 1975.9 cells/µl, monocytes: 109.95 ± 156.9 cells/µl, N/L ratio: 1.03 ± 0.66, total proteins 7 ± 0.39 gr/dl, albumin 4.48 ± 0.18 gr/dl, globulin 2.51 ± 0.43 gr/dl, A/G ratio 1.7 ± 0.71, fibrinogen 206 ± 107.6 gr /dl. Conclusions. Significant differences were established in the mean of total leukocytes, differentials and fibrinogen compared to other equids; these values were closer to those found in Catalans donkeys. In contrast, there were no differences in the values of leukocytes, neutrophils and lymphocytes with other young mules. The mean values of total proteins, albumin and globulin, were found to be closer to the values reported in horses. The results obtained show the importance of research on haematological parameters in mules according to breed, age, and management.

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Author Biographies

Ángela Ariza Suárez, Clínica de Grandes Animales Francisco de Asís - Fundación Universitaria Juan de Castellanos

Grupo de Investigación IRABI - Línea de Sanidad Animal. Clínica de Grandes Animales Francisco de Asís. Programa de Medicina Veterinaria. Facultad de Ciencias Agrarias y Ambientales. Fundación Universitaria Juan de Castellanos, Tunja-Boyacá, Colombia. 

Magnolia Maria Rosas Gutierrez, Clínica de Grandes Animales Francisco de Asís - Fundación Universitaria Juan de Castellanos

Grupo de Investigación IRABI - Línea de Sanidad Animal. Clínica de Grandes Animales Francisco de Asís. Programa de Medicina Veterinaria. Facultad de Ciencias Agrarias y Ambientales. Fundación Universitaria Juan de Castellanos, Tunja-Boyacá, Colombia. 

Juan Camilo Calderón García, Policía Nacional de Colombia

Policía Nacional de Colombia. Dirección de Carabineros y Seguridad Rural. Grupo Remonta y Veterinaria. Grupo Investigación ESREY Santa Rosa de Viterbo – Boyacá, Colombia. 

José Alejandro Prieto Lux, Clínica de Grandes Animales Francisco de Asís - Fundación Universitaria Juan de Castellanos

Grupo de Investigación IRABI - Línea de Sanidad Animal. Clínica de Grandes Animales Francisco de Asís. Programa de Medicina Veterinaria. Facultad de Ciencias Agrarias y Ambientales. Fundación Universitaria Juan de Castellanos, Tunja-Boyacá, Colombia. 

References

Ribeiro AL, Severino RS, Guerra RR, Favaron PO, Tommasi Junior H.L.P, Ricci REG, Franciolli ALR, Facciotti PR & Bombonato PP. Biometria de pontes de miocárdio em muares (Equus caballus x Equus asinus - Linnaeus 1758). Revista Biotemas 2009; 22: 177-184, 2009. https://periodicos.ufsc.br/index.php/biotemas/article/viewFile/19560/17930

Herrera Y, Pérez J, Rugeles C. Perfil proteico en mulares (Equus mulus) en condiciones naturales en el departamento de Córdoba, Colombia Multifaceted profile in of mules (Equus mulus) in natural conditions in the department of Córdoba, Colombia. Rev Colombiana Cienc Anim 2018; 10 (2): 173-178. http://dx.doi.org/10.24188/recia.v10.n2.2018.625

Aros K, Carrasco J, Briones R, Tadich T. Haematological and serum biochemical reference values for urban-workings equines in Chile. Austral J Vet Sci 2017; 1: 27-33. http://dx.doi.org/10.4067/s0719-81322017000100027

Izurieta, J., Luna, D., Cedeño, Y., & Chacha, S. Determinacion de los valores de referencia en el hemograma de caballos nacidos o criados a mas de 3000 m.s.n.m. en la sierra centro norte Ecuatoriana. La Granja: Rev de Cienc de la Vida 2017, 25 (1), 62-70. doi: http://dx.doi.org/10.17163/lgr.n25.2017.06

Pereira E, de Araujo AL, Cunha LA, Barcellos MP, Spadeto O. & Coelho CS. Eritrograma em muares submetidos à prova de resistência de 100 Km Rev. Bras Med Vet 2014; 36 (2): 226-230. http://rbmv.org/index.php/BJVM/article/download/499/375

Buitrago JA, Mora JP & Parra ZL. Valores de referencia para el recuento leucocítico en mulares del suroeste Antioqueño. Revista Sinergia 2018, Edición 4, 160-173. http://sinergia.colmayor.edu.co/ojs/index.php/Revistasinergia/article/view/63

Kowal R.J., Almosny N.R., Cascardo B., Summa R.P. & Cury L.J. Avaliação dos valores hematológicos em cavalos (Equus caballus) da raça Puro-Sangue-Inglês (PSI) submetidos a teste de esforço em esteira ergométrica. Rev Bras Cien Vet 2006; 13: 25-31. http://dx.doi.org/10.4322/rbcv.2014.261

Pritchard J.C., Burn C.C., Barr A.R.S. & Whay H.R. Haematological and serum biochemical reference values for apparently healthy working horses in Pakistan. Res Vet Sci 2009; 87: 389-395. https://doi.org/10.1016/j.rvsc.2009.05.003

Simenew K, Gezahegne M, & Getachew, M, Wondyefraw, M., Alemayehu L & Eyob, I. Reference Values of Clinically Important Physiological, Hematological and Serum Biochemical Parameters of Apparently Healthy Working Equids of Ethiopia. Global Veterinaria 2011; 7: 01-06. https://www.researchgate.net/publication/215871791

Folch P, Jordana J, Cuenca R. Reference ranges and the influence of age and sex on haematological values of the endangered Catalonian donkey. Vet Jrnl 1997; 154: 163–168. doi:10.1016/S10900233(97)80054-8.

García ME. Caracterización morfológica, hematológica y bioquímica clínica en cinco razas asnales españolas para programas de conservación. Tesis Doctoral. Departamento de Ciencia Animal y de los alimentos. Facultad de Veterinaria. Universidad Autónoma de Barcelona. Bellaterra 2006; 261 p. https://ddd.uab.cat/record/38317

Mclean A. & Wang W. Pilot study comparing hematologic and serum biochemical parameters in healthy horses (Equus caballus) and Mules. J. of equine Vet Sci 2013; 33, 321-339. https://doi.org/10.1016/j.jevs.2013.03.079

Herrera Y, Rugeles C &Vergara O. Perfil hematológico del burro del burro criollo (Equus asinus) colombiano. Rev Colom Cienc Anim 2017; 9 (2): 158-163. https://revistas.unisucre.edu.co/index.php/recia/article/view/553

Etana K.M., Jenbere T.S., Bojia E. & Negussie H. Determination of reference hematological and serum-biochemical values for working donkeys of Ethiopia. Vet Res 2011; 4: 90-94. doi: 10.3923/vr.2011.90.94.

McLean A, Varnum A, Ali A, Heleski C, & Navas FJ. Comparing and Contrasting Knowledge on Mules and Hinnies as a Tool to Comprehend Their Behavior and Improve Their Welfare. Animals 2019; 9: 488. doi: 10.3390/ani9080488

Lagos J & Tadich TA (2019) Hematological and Biochemical Reference Intervals for Mules in Chile. Front Vet Sci 2019; 6: 400. doi: 10.3389/fvets.2019.00400

Brosnahan M. Genetics, evolution, and physiology of donkeys and mules. Vet Clin Equine 2019; 35: 457–467 https://doi.org/10.1016/j.cveq.2019.08.003

Mclean A., Wang W., Navas-Gonzalez F., & Rodrigues J. Reference intervals for hematological and blood biochemistry reference values in healthy mules and hinnies. Comparative Clinical pathology 2016; 25 (3): 2276-3 doi: 10.1007/s00580-016-

Sandoval W & Oliver O. Propuesta de valores normales de hematología y química sanguínea de equinos y bovinos adultos sanos pertenecientes a clubes y hatos con remisión de pacientes a la clínica de grandes animales. Tesis de pregrado. Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional. Bogotá; 2002.

Satué K, Hernández A, Muñoz A. (2012). Physiological Factors in the Interpretation of Equine Hematological Profile, Hematology - Sci and Prac 2012; 573-596. IntechOpen, DOI: 10.5772/38961. https://www.intechopen.com/chapters/31178

Welles E. Interpretación of equine leukocyte responses Chapter 47. In Weiss J & Wardope J. Schalm´s Veterinary Hematology. 2010; 6th ed. Wiley-Blackwell 314-320 p. ISBN 978-0-8138-1798-9

Friedrichs K, Harr KE, Freeman K, Szladovits B, Walton R, et al. 2012. ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics. Vet Clin Pathol 2012; 41: 441-453. doi:10.1111/vcp.12006.

Crisman MV, Scarrat Wk, Zimmerman KL. Blood proteins and inflammation in the horse. Vet Clin Equine 2008; 24: 285-297. doi:10.1016/j.cveq.2008.03.004

Dias D. de C. Hematologia e bioquímica sérica em muares. Tesis de Maestria., Facultad de Medicina Veterinaria y Zootecnia. Universidad de Sao Paulo 2014; 103 p. doi:10.11606/D.10.2014.tde-28072014-153256.

Medeiros AP, Dos Anjos ST, Franciscato C, Segala LS, Merini LP. Valores hematológicos, proteínas plasmáticas totais e fibrinogënio do cavalo croulo- suas variações em relação ao sexo, idade e manejo. Acta Scientiae Vet 2006, 34 (3): 275 -279. doi: 10.22456/1679-9216.15462

Goodrich EL & Behlink-Kelly E. Clinical Pathology of Donkeys and Mules. Vet Clin Equine 35 (2019): 433–455. https://doi.org/10.1016/j.cveq.2019.08.002

Published

2021-11-09

How to Cite

Ariza Suárez, Ángela, Rosas Gutierrez, M. M. ., Calderón García, J. C., & Prieto Lux, J. A. (2021). Leukogram, total and differentiated proteins in young Equus mulus from the police in Boyacá (Colombia). CES Medicina Veterinaria Y Zootecnia, 16(2), 30–46. https://doi.org/10.21615/cesmvz.6313

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ARTÍCULO ORIGINAL DE INVESTIGACIÓN
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