Ensayo <i>in vitro</i> de extractos de <i>Lippia graveolens</i> y <i>Lippia alba</i> contra <i>Aeromonas</i> spp aisladas de tilapia (<i>Oreochromis niloticus</i>)

Autores/as

DOI:

https://doi.org/10.21615/cesmvz.6452

Palabras clave:

Aeromonas hydrophila, Aeromonas sobria, actividad antimicrobiana, acuicultura

Resumen

El presente estudio evaluó la actividad antimicrobiana de extractos y aceites esenciales de Lippia graveolens y Lippia alba, frente a nueve cepas de Aeromonas spp., aisladas de Oreochromis niloticus. Los extractos crudos se obtuvieron por la técnica de percolación y a través de la extracción de CO2 supercrítico, mientras que los aceites esenciales se realizaron mediante la técnica de hidrodestilación empleando un equipo Clevenger. La actividad antimicrobiana para cada extracto y aceite esencial se verificó mediante el método de difusión en disco a las 24 horas. El aceite esencial de L. graveolens mostró mayor efectividad para inhibir patógenos bacterianos de peces (100%), en comparación con los extractos. Se obtuvo un diámetro de inhibición que osciló entre 25,20 a 36,94 mm. Los extractos de fluido supercrítico y de la técnica de percolación con acetato de etilo y ciclohexano presentaron la misma efectividad (77,78%). El extracto crudo obtenido con etanol al 95% mostró efecto antimicrobiano limitado (22,22%), presentando el menor halo de inhibición (8,34 y 9,57 mm). Por otro parte, L. alba mostró menor actividad antibacteriana. El aceite esencial inhibió únicamente el 66,67% de las bacterias patógenas, presentando un halo de inhibición que oscila entre 10,68 a 16,29 mm. El resultado de este estudio indica que los aceites esenciales de L. graveolens y L. alba son una alternativa prometedora para el control del crecimiento de Aeromonas spp.

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Biografía del autor/a

Josue Garcia, Instituto Tecnológico de Costa Rica - Universidad Nacional - Universidad Estatal a Distancia - Universidad de San Carlos de Guatemala

Doctorado en Ciencias Naturales para el Desarrollo (DOCINADE), Instituto Tecnológico de Costa Rica, Universidad Nacional, Universidad Estatal a Distancia, Costa Rica. Centro de Estudios del Mar y Acuicultura, Universidad de San Carlos de Guatemala, Guatemala, Guatemala.

Juan Francisco Pérez-Sabino, niversidad de San Carlos de Guatemala

Escuela de Química, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Guatemala, Guatemala. 

Susana Mendoza-Elvira, Universidad Nacional Autónoma de México

Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Estado de México, México. 

Antonio Ribeiro da Silva, Universidad de Río de Janeiro

Instituto de Pesquisas de Productos Naturales, Universidad de Río de Janeiro, Brasil. 

Juan Ulloa-Rojas, Universidad Nacional

Escuela de Ciencias Biológicas. Universidad Nacional, Heredia, Costa Rica. 

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Publicado

2022-08-11

Cómo citar

Garcia, J., Pérez-Sabino, J. . F., Mendoza-Elvira, S., Ribeiro da Silva, A., & Ulloa-Rojas, J. . (2022). Ensayo <i>in vitro</i> de extractos de <i>Lippia graveolens</i> y <i>Lippia alba</i> contra <i>Aeromonas</i> spp aisladas de tilapia (<i>Oreochromis niloticus</i>). CES Medicina Veterinaria Y Zootecnia, 17(1), 28–46. https://doi.org/10.21615/cesmvz.6452

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ARTÍCULO ORIGINAL DE INVESTIGACIÓN