Olive oil as an alternative to boar semen cryopreservation

Autores/as

  • Estela Ferandes e Silva Universidad CES
  • Tainã Figueiredo Cardoso Universidade Federal do Rio Grande
  • Antonio Sergio Varela Junior Universidade Federal do Rio Grande
  • Fabiana Lemos Goulart Dutra Universidade Federal de Pelota
  • Fábio Pereira Leivas Leite Universidade Federal de Pelota
  • Carine Dahl Corcini Universidade Federal de Pelota

Resumen

The aim of this study was to evaluate different concentrations of olive oil for cryopreservation of boar semen. A total of 21 eyaculates of 18 males with motility ≥70% were used. For freezing, the semen was diluted in treatments: control (Cont–egg yolk), “Koroneiki” oil in egg yolk at 0.25% (A025), 0.50% (A050), 0.75% (A075) and 1.0% (A10). Straws with 5.107 sperm/ml were frozen and stored in liquid nitrogen. Thawing was done at 37ºC for 30 seconds and motility, mitochondrial functionality, DNA integrity, oocite penetration rate and the number of sperm per oocite (in the in vitro penetration trial) were evaluated. The variables were compared using the Kruskal-Wallis test. Even though no statistical difference was found between control and treatments containingolive oil (p>0.05), the treatment with 0.25% oil concentration showed tendences towards sperm protection, outperforming the controls in mitochondrial functionality and preservation of the fertilizing capabilities in the in vitro penetration trial. This is why, olive oil could represent an alternative to help cryopreservation of boar semen.

 

DOI: http://dx.doi.org/10.21615/cesmvz.11.1.1

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Biografía del autor/a

Estela Ferandes e Silva, Universidad CES

Universidade Federal do Rio Grande – Campus Carreiros, Av. Itália km 8 Bairro Carreiros - 475 - Aeroporto, Rio Grande

Tainã Figueiredo Cardoso, Universidade Federal do Rio Grande

Universidade Federal do Rio Grande – Campus Carreiros, Av. Itália km 8 Bairro Carreiros - 475 - Aeroporto, Rio Grande

Antonio Sergio Varela Junior, Universidade Federal do Rio Grande

Universidade Federal do Rio Grande – Campus Carreiros, Av. Itália km 8 Bairro Carreiros - 475 - Aeroporto, Rio Grande

Fabiana Lemos Goulart Dutra, Universidade Federal de Pelota

Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brasil

Fábio Pereira Leivas Leite, Universidade Federal de Pelota

Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brasil

Carine Dahl Corcini, Universidade Federal de Pelota

Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brasil

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Publicado

2016-05-16

Cómo citar

Ferandes e Silva, E., Figueiredo Cardoso, T., Varela Junior, A. S., Goulart Dutra, F. L., Leivas Leite, F. P., & Dahl Corcini, C. (2016). Olive oil as an alternative to boar semen cryopreservation. CES Medicina Veterinaria Y Zootecnia, 11(1), 8–14. Recuperado a partir de https://revistas.ces.edu.co/index.php/mvz/article/view/3826

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