Aislamiento y capacidad de osteodiferenciación de las células madre provenientes del ligamento periodontal y pulpa dental
Resumen
Introducción y objetivo: Los tejidos dentales contienen células madre mesenquimales con diversas capacidades de diferenciación. El objetivo de este estudio fue comparar la capacidad de osteodiferenciación de células madre de pulpa dental y ligamento periodontal cultivadas con diferentes medios de osteodiferenciación.
Materiales y métodos: Se obtuvieron 24 muestras de tejido pulpar y 30 de ligamento periodontal de terceros molares, se aislaron y se cultivaron con 5 condiciones diferentes de osteoinducción. La osteodiferenciación fue evaluada mediante RT-PCR y Rojo Alizarin.
Resultados: Las células aisladas fueron caracterizadas mediante citometría de flujo. Se observó una mayor expresión de Colágeno tipo I para células de pulpa dental, mientras que las células de ligamento periodontal expresaron mayores niveles de RUNX2. Las células Madre de ligamento periodontal presentaron mejor perfil de osteodiferenciación cuando fueron cultivadas en el medio 5,mientras que las células madre extraídas de la pulpa dental presentaron mejores características de osteodiferenciación en el medio de cultivo 4. La única diferencia entre estos dos medios fue la concentración de 50uM de Ácido Ascórbico para el medio 5 y 200uM de Ácido Ascórbico para el medio 4.
Conclusiones: Las células obtenidas de pulpa dental y ligamento periodontal mostraron características de células madre. La capacidad de osteodiferenciación de estas células fue dependiente de las concentraciones de los sustratos aplicados al cultivo.
Isolation and osteodifferentiation capacity of dental pulp and periodontal ligament stem cells
Introduction and objective: Dental tissues have mesenchymal stem cells with capacities to differentiate in several tissues. The aim of this research was to compare the ability of osteodifferentiation of stem cells isolated of dental pulp and periodontal ligament cultured with different media.
Materials and methods: 24 samples of pulp tissue and 30 samples of periodontal ligament were obtained of third molars. Stem cells isolated by the explant method were subjected to 5 osteodifferentiation conditions. Osteodifferentiation was evaluated by RT-PCR and Alizarin Red.
Conclusions: The cells obtained both pulp and ligament showed features of Stem Cells. The osteogenic capacity of cells was depending of the concentrations of the substrates applied.
Keywords: Stem Cells , Dental Pulp, Periodontal Ligament, osteodifferentiation
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