Diferencias en los polimorfismos de genes asociados al control del desarrollo folicular entre búfalos (Bubalus bubalis) y vacunos (Bos indicus)

Jesús Alfredo Berdugo Gutiérrez, Ariel Marcel Tarazona-Morales, José Julian Echeverry-Zuluaga, José Luis Buitrago-Castaño, Albeiro Lopez-Herrera

Resumen


Se han encontrado asociaciones entre polimorfismos genéticos y  parámetros reproductivos, adicioalmente este abordaje permitiría proponer  explicaciones a los fenómenos estudiados. FSHr, InhA, AMH y AMHr son genes asociados al desarrollo folicular, que tienen una alta homologia  y la misma función en bovinos. El objetivo del presente trabajo fue evaluar en bovinos muy relacionados si se encuentran   algunos polimorfismos asociados a parámetros reproductivos reportados en otras especies. Durante el  2018, en el Magdalena Medio Colombiano fueron tomadas muestras de sangre anticoagulada de 50 búfalas y 50 vacas  cebú comercial (Bos indicus). Se registró la paridad, días abiertos, intervalo parto primer servicio y edad al primer parto de cada animal,  sin alteraciones anatómicas, con   edad y peso similar,  mantenidos en las mismas condiciones de alimentación y manejo. Se  extrajo el ADN por el método de Salting out y se evaluaron los polimorfismos de acuerdo con lo reportado en la literatura. Se compararon los datos usando la prueba Mann Whitney, se consideró significativo un valor de p<0,05. Se encontró que los búfalos tienen menores niveles de AMH, edad al primer parto, intervalo ente partos que las vacas (p<0.001), mayor paridad (p=0.005). No se confirmaron los polimorfismos reportados en holstein y en humanos en ninguna de las muestras y especies analizadas. No confirmar los polimorfismos hallados por otros autores en bovinos de otras razas y especies, aún mostrando  que  tienen diferentes  parámetros reproductivos ,  muestra la necesidad de replantear el abordaje del estudio de los asociación de los fenómenos reproductivos y genéticos.

 

Differences in polymorphisms of genes associated with follicle development control between buffaloes (Bubalus bubalis) and cattle (Bos indicus)

Associations have been found between genetic polymorphisms and reproductive parameters, this approach additionally would allow us to propose explanations to the phenomena studied. FSHr, InhA, AMH and AMHr are genes associated with follicular development, which have a high homology and the same function in bovines. The objective of this work was to evaluate if some polymorphisms described in other species associated with reproductive parameters are found in two closely related bovines. During 2018, in the Colombian Magdalena Medio anticoagulated blood samples from 50 buffaloes (Bubalus bubalis) and 50 commercial zebu cows (Bos indicus) were taken. All animals without anatomical abnormalities and similar weight and age were taken and the. parity, intercalving period and age at first calving were also recorded. DNA were extracted using Salting out method and the reported polymorphisms of the above mentioned genes were evaluated. Data were compared using Mann Whitney test and p<0.05 value were considered significant. Buffaloes have lower AMH levels, age at first calving, calving interval than Bos indicus cows (p<0.001) and higher parity (p=0.005). The polymorphisms reported in Holstein and humans were not confirmed in any of the samples and species analyzed. The results, are in some way parodoxical because there are differences in reproductive parameters but nothing different in the studies genes. It shows the need to rethink the approach of the study of the association of reproductive and genetic phenomena.

Keywords: AMH, AMHr, Buffaloes, FSHr, InhA, Polymorphisms.

 

Diferenças nos polimorfismos genéticos associados ao controle do desenvolvimento folicular entre búfalos (Bubalus bubalis) e bovinos (Bos indicus)

Foram encontradas associações entre polimorfismos genéticos e parâmetros reprodutivos; essa abordagem também permitiria propor explicações para os diferentes fenômenos estudados. FSHr, InhA, AMH e AMHr são genes associados ao desenvolvimento folicular, que apresentam alta homologia e a mesma função em bovinos. O objetivo do presente trabalho foi avaliar em bovinos intimamente relacionados se alguns polimorfismos associados a parâmetros reprodutivos relatados em outras espécies. Em 2018, 50 vacas de búfalo (Bubalus bubalis) e 50 de zebu comerciais (Bos indicus) foram amostradas na Magdalena Medio colombiana. Foram registradas paridade, dias abertos, intervalo de nascimento do primeiro serviço e idade no primeiro nascimento de cada animal, sem alterações anatômicas, com idade e peso semelhantes, mantidas nas mesmas condições de alimentação e manuseio. O DNA foi extraído pelo método Salting out e os polimorfismos foram avaliados de acordo com o relatado na literatura. Os dados foram comparados pelo teste de Mann Whitney, sendo considerado significativo um valor de p <0,05. Verificou-se que os búfalos apresentam níveis mais baixos de AMH, idade do primeiro nascimento, intervalo entre os nascimentos que as vacas (p <0,001), maior paridade (p = 0,005). Os polimorfismos relatados em Holstein e em humanos não foram confirmados em nenhuma das amostras e espécies analisadas. A não confirmação dos achados em bovinos de outras raças e espécies, embora as espécies possuam diferentes parâmetros reprodutivos, mostra a necessidade de repensar a abordagem do estudo da associação dos fenômenos reprodutivos e genéticos.

Palavras-chave: AMH, AMHr, buffalo, FSHr, InhA, polimorfismos.


Palabras clave


AMH, AMHr, Búfalos, FSHr, InhA, Polimorfismos.

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