Efecto de los extractos vegetales de Jatropha curcas y Anonna muricata sobre teleoginas de la garrapata común del ganado Ripichephalus (Boophilus) microplus bajo condiciones de laboratorio

Juan Pablo Lopera Vélez, Guillermo León Hernández, Pablo Andrés Guzmán, Carlos Andrés Escobar Guerra

Resumen


La garrapata común (Rhipicephalus (B.) microplus) del ganado es considerada el ectoparásito más importante en la ganadería. El uso repetitivo y tradicional de acaricidas comerciales se ha convertido en un serio problema en cuanto a la resistencia adquirida por esta garrapata. Por esta razón, el control biológico ha surgido como una alternativa prometedora en el control de este ectoparásito. En este estudio se evalúa el efecto de los extractos vegetales de dos plantas, J. curcas y A. muricata, sobre el índice de producción de huevos y la eficiencia reproductiva de R (B.) microplus bajo condiciones de laboratorio. Cada extracto fue evaluado a cinco concentraciones (entre 0.1 y 5%), usando controles negativo y positivo para su comparación. Ambas plantas mostraron actividad biológica, sin embargo, A. muricata tuvo un efecto mayor comparado con J. curcas, inclusive a concentraciones bajas (0,5 y 1 %). Por el contrario, J. curcas mostró solo un efecto moderado en concentraciones altas (5%). En conclusión, los resultados obtenidos en laboratorio indican que los extractos de A. muricata son promisorios para controlar la garrapata común del ganado.

 

Effect of plant extracts of jatropha curcas and Anonna muricata on engorged females of common cattle tick Ripichephalus (Boophilus) microplus under laboratory conditions

Rhipicephalus (B.) microplus or common cattle tick is considered the most important ectoparasite of livestock. Traditional and repetitive control of this tick through the use of conventional products has become a serious problem regarding tick’s resistance. For this reason, biological control has emerged as a very promising alternative to control this tick. In the present study, an experiment was designed to assess the effect of plant extracts of J. curcas y A. muricata on Production eggs Index (IPH) and estimated reproduction (ER) of R (B) microplus under laboratory conditions. Each plant extract was assessed in five different concentrations (between 0.1 and 5%); a positive and negative control was used for comparison. Both plants showed biological activity, however, A. muricata showed significantly effect on engorged females of the cattle tick compared with J. curcas, even at lower concentrations (0.5, and 1 %). In conclusion, the results obtained indicate that A. muricata’s seed extracts are promissory to biocontrol the common cattle tick under laboratory conditions.

Keywords: estimated reproduction (ER), Production eggs Index (IPH), Rhipicephalus (B.) microplus, vegetal extracts.

 

Efeito de extractos de plantas de Jatropha curcas e Anonna muricata em teleogines do carrapato gado comum Ripichephalus (Boophilus) microplus sob condições de laboratório.

O carrapato comum (Rhipicephalus (B.) microplus) do gado é considerado o ectoparasita mais importante da pecuária. O uso repetitivo e tradicional de inseticidas comerciais se converteu em um sério problema quanto a resisitência adquirida por esse carrapato. Por essa razão, o controle biológico surgiu como uma alternativa promissora no controle deste ectoparasita. Esse estudo avalia o efeito dos extratos vegetais das plantas, J. curcas e A. muricata, sobre o índice de produção de ovos e a eficiência reprodutiva de R (B.) microplus segundo condições de laboratório. Cada  extrato foi avaliado em 5 concentrações (entre 0,1 e 5%), usando controles negativo e positivo para sua comparação. Ambas as plantas mostraram atividade biológica, porém,A. muricata teve um efeito maior comparado com  J. curcas, inclusive as concentrações baixas (0,5 y 1 %). Pelo contrário, J. curcas mostrou  só um efeito moderado nas concentrações altas (5%). Em conclusão, os resultados obtidos no laboratório indicam que os extratos de  A. muricata são promissores para controlar o carrapato comum do gado.

Palabras-chave: eficiência reprodutiva (ER), extractos de plantas, Rhipicephalus (B.) microplus, taxa de produção de ovos (TPO).

DOI: http://dx.doi.org/10.21615/cesmvz.12.1.2


Palabras clave


eficiencia reproductiva (ER), extractos vegetales, índice de producción de huevos (IPH), Rhipicephalus (B.) microplus

Citas


Achten, W.M.J., L. Verchot, Y.J. Franken, E. Mathijs, V.P. Singh, R. Aerts, and B. Muys. 2008. “Jatropha Bio-Diesel Production and Use.” Biomass and Bioenergy 32 (12): 1063–84. doi:10.1016/j.biombioe.2008.03.003.

Aiyelaagbe, Olapeju O., Amao A. Hamid, Ernesto Fattorusso, Orazio Taglialatela-Scafati, Heinz C. Schröder, and Werner E. G. Müller. 2011. “Cytotoxic Activity of Crude Extracts as Well as of Pure Components from Jatropha Species, Plants Used Extensively in African Traditional Medicine.” Evidence-Based Complementary and Alternative Medicine: eCAM 2011: 134954. doi:10.1155/2011/134954.

Álvarez, Víctor, Jorge Loaiza, Roberto Bonilla, and Mariano Barrios. 2006. “Control in Vitro de Garrapatas (Boophilus Microplus; Acari: Ixodidae) Mediante Extractos Vegetales.” Revista de Biología Tropical 56 (1). doi:10.15517/rbt.v56i1.5525.

Amariles, Santiago, Carlos García-Pajón, and Gabriel Parra-Henao. 2013. “Actividad Insecticida de Extractos Vegetales Sobre Larvas de Aedes Aegypti, Diptera: Culicidae.” CES MEDICINA 27 (2): 193–203. http://www.scielo.org.co/pdf/cesm/v27n2/v27n2a07.pdf

Badrie, Neela, and Alexander Schauss. 2009. “Soursop (Annona Muricata L.): Composition, Nutritional Value, Medicinal Uses, and Toxicology.” In Bioactive Foods in Promoting Health: Fruits and Vegetables, 621–43. Oxford Academic Press. https://www.researchgate.net/publication/236834878_Soursop_Annona_muricata_L_Composition_nutritional_value_medicinal_uses_and_toxicology

Bevilaqua, Claudia M. L., Selene M. Morais, Lyeghyna K. Andrade Machado, Ana Lourdes F. Camurça-Vasconcelos, Claudio C. Campello, Wesley L. C. Ribeiro, and Mayara de A. Mesquita. 2011. “Anthelmintic Activity of Jatropha Curcas L. Seeds on Haemonchus Contortus.” Veterinary Parasitology 182 (2–4): 259–63. doi:10.1016/j.vetpar.2011.04.010.

Bisen, S, Mandal S.C, and Sanyal P.K. 2011. “Effect of Some Phytotherapeutic Agents on Egg Production of Rhipicephalus (Boophilus) Microplus.” Indian Journal of Animal Research 45 (4): 289–94. https://www.cabdirect.org/cabdirect/abstract/20123156145

Borges, Lígia Miranda Ferreira, Lorena Alessandra Dias de Sousa, and Carolina da Silva Barbosa. 2011. “Perspectives for the Use of Plant Extracts to Control the Cattle Tick Rhipicephalus (Boophilus) Microplus.” Revista Brasileira De Parasitologia Veterinária = Brazilian Journal of Veterinary Parasitology: Órgão Oficial Do Colégio Brasileiro De Parasitologia Veterinária 20 (2): 89–96. https://www.ncbi.nlm.nih.gov/pubmed/21722481

Cabrera, Darwin, Roger Rodríguez, and José Rosado. 2008. “Evaluación de la resistencia a la cipermetina en cepas de campo de Boophilus microplus obtenidas de ranchos bovinos del estado de Yucatán, México.” Técnica Pecuaria en México 46 (4): 439–48. http://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/1793/1787

Castro-Janer, E., J. R. Martins, M. C. Mendes, A. Namindome, G. M. Klafke, and T. T. S. Schumaker. 2010. “Diagnoses of Fipronil Resistance in Brazilian Cattle Ticks (Rhipicephalus (Boophilus) Microplus) Using in Vitro Larval Bioassays.” Veterinary Parasitology 173 (3–4): 300–306. doi:10.1016/j.vetpar.2010.06.036.

Castro-Janer, E., L. Rifran, P. González, J. Piaggio, A. Gil, and T. T. S. Schumaker. 2010. “Rhipicephalus (Boophilus) Microplus (Acari: Ixodidae) Resistance to Fipronil in Uruguay Evaluated by in Vitro Bioassays.” Veterinary Parasitology 169 (1–2): 172–77. doi:10.1016/j.vetpar.2009.12.017.

Cavé, A., B. Figadère, A. Laurens, and D. Cortes. 1997. “Acetogenins from Annonaceae.” In Fortschritte Der Chemie Organischer Naturstoffe Progress in the Chemistry of Organic Natural Products, by A. Cavé, D. Cortes, B. Figadère, A. Laurens, and G. R. Pettit, edited by W. Herz, G. W. Kirby, R. E. Moore, W. Steglich, and Ch. Tamm, 70:81–288. Vienna: Springer Vienna. http://www.springerlink.com/index/10.1007/978-3-7091-6551-5_2.

Devappa, Rakshit K., Miguel A. Angulo-Escalante, Harinder P.S. Makkar, and Klaus Becker. 2012. “Potential of Using Phorbol Esters as an Insecticide against Spodoptera Frugiperda.” Industrial Crops and Products 38 (July): 50–53. doi:10.1016/j.indcrop.2012.01.009.

Devappa, Rakshit K., Harinder P. S. Makkar, and Klaus Becker. 2010a. “Jatropha Toxicity—A Review.” Journal of Toxicology and Environmental Health, Part B 13 (6): 476–507. doi:10.1080/10937404.2010.499736.

Devappa, Rakshit K., H.P.S. Makkar, and K. Becker. 2010b. “Optimization of Conditions for the Extraction of Phorbol Esters from Jatropha Oil.” Biomass and Bioenergy 34 (8): 1125–33. doi:10.1016/j.biombioe.2010.03.001.

Devappa, Rakshit K., Sanjay K. Rajesh, Vikas Kumar, Harinder P.S. Makkar, and Klaus Becker. 2012. “Activities of Jatropha Curcas Phorbol Esters in Various Bioassays.” Ecotoxicology and Environmental Safety 78 (April): 57–62. doi:10.1016/j.ecoenv.2011.11.002.

Djamin, Asmanizar, and Idris A. B. 2012. “Evaluation of Jatropha Curcas and Annona Muricata Seed Crude Extracts Against Sitophilus Zeamais Infesting Stored Rice.” Journal of Entomology 9 (1): 1–13. http://scialert.net/qredirect.php?doi=je.2012.13.22&linkid=pdf

Drummond, R. O., S. E. Ernst, J. L. Trevino, W. J. Gladney, and O. H. Graham. 1973. “Boophilus Annulatus and B. Microplus: Laboratory Tests of Insecticides.” Journal of Economic Entomology 66 (1): 130–33. https://academic.oup.com/jee/article-abstract/66/1/130/2211260/Boophilus-annulatus-and-B-microplus-Laboratory?redirectedFrom=fulltext

Ferreira, L. E., P. M. N. Castro, A. C. S. Chagas, S. C. França, and R. O. Beleboni. 2013. “In Vitro Anthelmintic Activity of Aqueous Leaf Extract of Annona Muricata L. (Annonaceae) against Haemonchus Contortus from Sheep.” Experimental Parasitology 134 (3): 327–32. doi:10.1016/j.exppara.2013.03.032.

Goel, Gunjan, Harinder P. S. Makkar, George Francis, and Klaus Becker. 2007. “Phorbol Esters: Structure, Biological Activity, and Toxicity in Animals.” International Journal of Toxicology 26 (4): 279–88. doi:10.1080/10915810701464641.

Juliet, Sanis, Reghu Ravindran, Sunil A Ramankutty, Ajith Kumar K Gopalan, Suresh N Nair, Amithamol K Kavillimakkil, Amitabh Bandyopadhyay, Ajay Kumar S Rawat, and Srikanta Ghosh. 2012. “Jatropha Curcas (Linn) Leaf Extract –a Possible Alternative for Population Control of Rhipicephalus(Boophilus) Annulatus.” Asian Pacific Journal of Tropical Disease 2 (3): 225–29. doi:10.1016/S2222-1808(12)60051-6.

Kaaya, Godwin P. 1992. “Non-Chemical Agents and Factors Capable of Regulating Tick Populations in Nature: A Mini Review.” International Journal of Tropical Insect Science 13 (4): 587–94. doi:10.1017/S1742758400016179.

Komansilan, Alfrits, Abdul L. Abadi, Bagyo Yanuwiadi, and David A. Kaligis. 2012. “Isolation and Identification of Biolarvicide from Soursop (Annona Muricata Linn) Seeds to Mosquito (Aedes Aegypti) Larvae.” International Journal of Engineering & Technology 12 (3): 28–32. http://www.ijens.org/vol_12_i_03/128503-5959-ijet-ijens.pdf

Kumar, Sachin, Souvik Paul, Anil Kumar Sharma, Rinesh Kumar, Shashi Shankar Tewari, Pallab Chaudhuri, D.D. Ray, Ajay Kumar Singh Rawat, and Srikant Ghosh. 2011. “Diazinon Resistant Status in Rhipicephalus (Boophilus) Microplus Collected from Different Agro-Climatic Regions of India.” Veterinary Parasitology 181 (2–4): 274–81. doi:10.1016/j.vetpar.2011.04.030.

López, Elkin, Gustavo López, and Sergio Orduz. 2009. “Control de la garrapata Boophilus microplus con Metarhizium anisopliae, estudios de laboratorio y campo.” Revista Colombiana de Entomología 35 (1): 42–46. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-04882009000100008

López-Valencia, Gustavo. 2013. “Aspectos Generales Sobre Las Garrapatas, Distribución, Morfología, Biología Y Control.” In Productos Naturales Contra Parásitos Externos Del Ganado Bovino Y Ovino, Tales Como Mosca de Los Cuernos Y Garrapatas, 1sted., 15–29. Chile: Ediciones de la universidad de Magallanes. http://odin.ces.edu.co/Contenidos_Web/41029076.pdf

Muro, Francisco, Carlos Cruz-Vasquez, Manuel Fernández-Ruvalcaba, and Jorge Molina Torres. 2004. “Efecto repelente de extractos de Melinis minutiflora sobre larvas de la garrapata Boophilus microplus.” Veterinaria México 35 (2): 153–59. http://www.medigraphic.com/pdfs/vetmex/vm-2004/vm042g.pdf

Pandey, Vimal Chandra, Kripal Singh, Jay Shankar Singh, Akhilesh Kumar, Bajrang Singh, and Rana P. Singh. 2012. “Jatropha Curcas: A Potential Biofuel Plant for Sustainable Environmental Development.” Renewable and Sustainable Energy Reviews 16 (5): 2870–83. doi:10.1016/j.rser.2012.02.004.

Parra-Henao, Gabriel, Carlos Mario Garcia Pajón, and Jose Miguel Cotes Torres. 2007. “Actividad insecticida de extractos vegetales sobre Rhodnius prolixus y Rhodnius pallescens (Hemiptera: Reduviidae).” Boletín de Malariología y Salud Ambiental 47: 125–37. http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S1690-46482007000100011

Ragasa, Consolacion Y, Geneveve Soriano, Oscar B Torres, Ming-Jaw Don, and Chien-Chang Shen. 2012. “Acetogenins from Annona Muricata.” Pharmacognosy Journal 4 (32): 32–37. doi:10.5530/pj.2012.32.7.

Rakshit, K.D., J. Darukeshwara, K. Rathina Raj, K. Narasimhamurthy, P. Saibaba, and S. Bhagya. 2008. “Toxicity Studies of Detoxified Jatropha Meal (Jatropha Curcas) in Rats.” Food and Chemical Toxicology 46 (12): 3621–25. doi:10.1016/j.fct.2008.09.010.

Ren, Qiaoyun, Zhijie Liu, Guiquan Guan, Ming Sun, Miling Ma, Qingli Niu, Youquan Li, et al. 2012. “Laboratory Evaluation of Virulence of Chinese Beauveria Bassiana and Metarhizium Anisopliae Isolates to Engorged Female Rhipicephalus (Boophilus) Microplus Ticks.” Biological Control 63 (2): 98–101. doi:10.1016/j.biocontrol.2012.07.002.

Robledo-Reyes, Paulo, Ranulfo González, Gloria Jaramillo, and Jaime Restrepo. 2008. “Evaluación de la toxicidad de acetogeninas anonáceas sobre ninfas de Periplaneta americana l. (Dictyoptera: Blattidae).” Boletín del Museo de Entomología de la Universidad del Valle 9 (1): 54–61. http://entomologia.univalle.edu.co/boletin/6Robledo.pdf

Rossini, Carmen. 2013. “Medidas de control contra la garrapata común del ganado.” In Productos naturales contra parásitos externos del ganado bovino y ovino, tales como mosca de los cuernos y garrapatas, 1sted., 30–42. Chile: Ediciones de la universidad de Magallanes. https://books.google.com.co/books/about/Productos_naturales_contra_parasitos_ext.html?id=xKZLnQAACAAJ&redir_esc=y

Rupprecht, J. Kent, Yu-Hua Hui, and Jerry L. McLaughlin. 1990. “Annonaceous Acetogenins: A Review.” Journal of Natural Products 53 (2): 237–78. doi:10.1021/np50068a001.

Santillán-Velazquez, Guadalupe, Froylán Ibarra-Velarde, Blas Flores Pérez, Margarita Romero-Avila, Yazmin Alcalá-Canto, Héctor Salgado-Zamora, and Yolanda Vera Montenegro. 2013. “In Vitro and in Vivo Efficacy of an Experimental Compound against Rhipicephalus (Boophilus) Microplus Ticks.” Pharmacology & Pharmacy 4 (1): 41–45. doi:10.4236/pp.2013.41005.

Solís-Fuentes, Julio A., María del Rosario Hernández-Medel, and María del Carmen Durán-de-Bazúa. 2011. “Soursop (Annona Muricata L.) Seeds, Therapeutic and Possible Food Potential.” In Nuts and Seeds in Health and Disease Prevention, 1045–52. Elsevier.

http://linkinghub.elsevier.com/retrieve/pii/B9780123756886101240.

Sousa, Orlando Vieira de, Glauciemar Del-Vechio Vieira, José de Jesus R. G. de Pinho, Célia Hitomi Yamamoto, and Maria Silvana Alves. 2010. “Antinociceptive and Anti-Inflammatory Activities of the Ethanol Extract of Annona Muricata L. Leaves in Animal Models.” International Journal of Molecular Sciences 11 (5): 2067–78. doi:10.3390/ijms11052067.


Texto completo: PDF

Licencia de Creative Commons

Este obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.