Linfoma difuso de células B rico en células T en un canino: Reporte de caso

Carmenza Janneth Benavides Melo, Carlos Alberto Chaves Velasquez, Juan Manuel Astaiza Martinez, Dario Antonio Vallejo Timaran, Anghy Marcela Ruiz Salazar

Resumen


Se reporta el caso de un Linfoma difuso de células grandes de células B rico en células T de bajo grado de comportamiento multicéntrico diagnosticado mediante evaluación histopatológica e inmunohistoquímica en un canino. En febrero de 2016 se remite al área de cátedra y servicio de patología veterinaria de la Universidad de Nariño, Pasto, Colombia, un canino hembra mestiza de ocho años de edad; el propietario reporta la presencia de dos masas en el cuello, decaimiento, anorexia y pérdida de peso. Se realiza biopsia por aspiración con aguja fina (BACAF) de masas submandibulares y linfonodos poplíteos evidenciando proliferación neoplásica de células redondas de característica linfoide, por lo cual el propietario decidió realizar eutanasia y necropsia. Se tomaron muestras para realizar la técnica de inclusión en parafina y coloración de Hematoxilina – Eosina (H/E) de rutina, adicionalmente se emplearon anticuerpos inmunohistoquímicos para CD45, CD3 y CD79a DAKO® los cuales se aplicaron a médula ósea, nódulos linfáticos, piel y fueron contrastados con hematoxilina de Meyer. Los hallazgos de H/E e inmunohistoquímica corresponden a un linfoma difuso de células B rico en células T de bajo grado, de comportamiento multicéntrico con metástasis en páncreas, hígado, pulmón, intestino delgado, médula ósea y piel. Se concluye que el uso de marcadores inmunohistoquímicos demuestran ser una herramienta de gran utilidad para el diagnóstico de neoplasias linfoides al correlacionarlos con la evaluación clínica e histopatológica.

 

Canine diffuse lymphoma of B-cell rich in T-cell: a case report

Was reported a diffuse large cell lymphoma, both B cell and rich in T cell, low-grade behavior multicenter, diagnosed by histopathological and immunohistochemical evaluation. In February 2016 in the veterinary clinic "Carlos Martinez H." University of Nariño, Pasto, Colombia, entered a canine female, crossbreed, eight years old; owner reported the presence of two masses in neck, with decay, anorexia and weight loss. Was taken a sample fine-needle biopsy aspiration (FNA) of submandibular masses and popliteal lymph nodes, showing neoplastic proliferation of round cells of lymphoid feature, so the owner decided to perform euthanasia and necropsy. Were sampled for inclusion paraffin technique, and staining hematoxylin - eosin (H / E), further immunohistochemical antibodies to CD45, CD3 and CD79a DAKO® which were applied and were compared with hematoxylin-eosin finds. Findings belong to diffuse lymphoma diffuse of B-cell rich in low-grade T-cell with multicentric distribution with metastases in pancreas, liver, lung, small intestine, bone marrow and skin. In conclusion, use of immunohistochemically markers proves a useful tool for diagnosis of lymphoid neoplasms when the findings are correlated with clinical and histopathological evaluation.

Keywords: cytology, histopathology, immunohistochemistry, lymphoid neoplasia, dog.

 

Linfoma difuso de células B rico em células T em um canino: relato de caso

O caso de um linfoma difuso de células grande de células B rico em células T baixo grau comportamento multicêntrico diagnosticada por histopatológico e imuno-histoquímica em um canino é relatado. Em fevereiro de 2016, refere-se à área de ensino e serviço de patologia veterinária da Universidade de Nariño, Pasto, Colômbia, uma fêmea mestiça canino oito anos de idade; o proprietário relatou a presença de dois massas cervicais, deterioração, anorexia e perda de peso. Biópsia aspiração é executada com aspiração com agulha fina (FNA) de massas dos nodos linfáticos submandibulares poplítea e que mostram a proliferação neoplásica de células redondas recurso linfoide, ou que o proprietário decidiu eutanásia e necropsia. Foram tomadas amostras para inclusão em parafina técnica e hematoxilina - eosina (H/E), adicionalmente foi usado os anticorpos imuno-histoquímica CD45, CD3 e CD79a - DAKO® que foram aplicadas para a medula óssea, os nódulos linfáticos, e a pele a foram comparadas com a corante hematoxilina de Meyer. Os resultados de H/E e imuno-histoquímica para corresponder a um linfoma difuso de células grande de células B rico em células T baixo grau comportamento multicêntrico com metástase no pâncreas, fígado, pulmão, intestino delgado, medula óssea e pele. Conclui-se que o uso de marcadores imuno-histoquímica se revela uma ferramenta útil para o diagnóstico de neoplasias linfoides para correlacionar com a avaliação clínica e histopatológica.

Palavras-chave: citologia, histopatologia, imuno-histoquímica, neoplasia linfoide, cão

DOI: http://dx.doi.org/10.21615/cesmvz.12.1.5


Palabras clave


citología; histopatología; inmunostoquímica; neoplasia linfoide; perro.

Citas


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